Technology

Spatial in situ multiomics is an emerging field with tremendous potential to advance life sciences research, drug development, and clinical diagnostics.

Spatial in situ multiomics utilizes the massively multiplexed barcoding and high-resolution imaging to simultaneously detect tens to thousands of RNA, DNA, proteins, and therapeutic molecules in situ.

The underlying technology of our spatial in situ multiomics platform is powered by proprietary FISSEQ (fluorescent in situ sequencing). FISSEQ technology simplifies the preparation of in situ sequencing libraries in cell and tissue samples, providing standardized workflows that require minimal optimization and overcome nuisances such as sample mounting and autofluorescence.

  • RNA FISSEQ enables flexible targeted sequencing for measuring single-cell gene
    expression, splice variation, and expressed sequence variants.
  • DNA FISSEQ detects single-cell chromosomal conformation and structural variation,
    such as CNV.
  • Protein FISSEQ provides rich morphological context around RNA & DNA localizations.

Together these assays enable spatial multi-omic measurements within a single sample.

Spatial in situ Multiomics Workflow

1

Manage

2

Prep

3

Sequence

4

Analyze

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Bioinformatics Tools for Spatial in situ Multiomics

“Discovering an HIV cure requires high-resolution in situ sequencing of cellular immune neighborhoods and inflammatory landscapes to elucidate immune pathways driving viral persistence.”

Jacob D. Estes, PhD, Oregon Health State University

The ability to generate data with RNA, DNA, protein, and morphology within a single 3D volume is unprecedented, and it requires new and novel tools to visualize and analyze. We developed our cloudware to view the data as a volume, with the ability to turn off and on molecules and morphology features, pan and zoom, and select-regions of interest.

In this sample from Dr. Jacob Estes, explore virus transcripts and host response in the lymph tissue, choose genes of interest, and compare frequencies.

 

Featured Research Areas

Our technology has been developed to advance research, drug development and clinical diagnosis. As part of our initial product offering, we have validated applications in Oncology, Neuroscience and Infectious Disease. We will also offer a custom application.


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Oncology

Understanding the tumor microenvironment on a patient-by-patient basis will improve immunotherapies. With our technology, researchers can better understand T-cells, immune signaling, and driver mutations within the tumor microenvironment at sub-cellular resolution.

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Infectious Disease

Our initial technology was designed to advance our understanding of HIV biology. We cover a broad spectrum of immune host interactions, cell-typing, signaling and activation pathways, virus genome (RNA & DNA), and virus transcriptome all localized within the same FFPE or Fresh Frozen sample.

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Neuroscience

Creation of a comprehensive brain cell atlas will have enormous benefit to both basic neuroscience research and biomedical applications such as understanding and treating traumatic brain injury and neurodegenerative diseases.

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